Detection of Sheep-Associated Malignant Catarrhal Fever Virus Antibodies by Complement Fixation Tests.
نویسندگان
چکیده
منابع مشابه
Field validation of laboratory tests for clinical diagnosis of sheep-associated malignant catarrhal fever.
Until recently, sheep-associated malignant catarrhal fever (SA-MCF) was diagnosed mainly on the basis of clinical presentation and histopathological changes. Using clinically diagnosed field cases, we have evaluated a seminested PCR and a competitive inhibition enzyme-linked immunosorbent assay (CI-ELISA) and compared these assays in the diagnosis of SA-MCF in cattle with histopathology as a pr...
متن کاملDetection of sheep-associated malignant catarrhal fever from clinical cases in Ethiopian cattle.
Mucoid nasal discharge, loss of weight, decreased milk production, diarrhoea, salivation, dyspnoea, fever, lacrimation, bilateral corneal opacity and bloody urine were observed in cattle located in the Arbe Gona district of southern Ethiopia. The disease was associated with a high case fatality rate: diseased cattle died within four to five days after showing clinical signs. The clinical presen...
متن کاملDiscrimination between sheep-associated and wildebeest-associated malignant catarrhal fever virus by means of a single-tube duplex nested PCR.
A single-tube duplex nested polymerase chain reaction (sdn-PCR) was developed for the detection of and discrimination between ovine herpesvirus-2 (OvHV-2) and alcelaphine herpesvirus-1 (AIHV-1). These viruses respectively cause sheep- and wildebeest-associated malignant catarrhal fever (SA-MCF and WA-MCF). In the first step of the sdn-PCR, two primers with high annealing temperatures based on c...
متن کاملMalignant Catarrhal Fever
MCF is defined by the recognition of characteristic lymphoid cell accumulations in non-lymphoid organs, vasculitis, and T-lymphocyte hyperplasia in lymphoid organs, and can be caused by either of two gamma-herpesviruses. The alcelaphine herpesvirus-1 (AIHV-1), the natural host of which, the wildebeest, is infected inapparently, causes the disease in cattle in regions of Africa and various rumin...
متن کاملInvestigation of sheep-associated malignant catarrhal fever virus infection in ruminants by PCR and competitive inhibition enzyme-linked immunosorbent assay.
Development of control measures for the gammaherpesviral disease of cattle known as sheep-associated malignant catarrhal fever (SA-MCF) has been hampered by a lack of accurate diagnostic tests either for the causative virus or for antibody against that virus. A recently developed competitive-inhibition enzyme-linked immunosorbent assay (CI-ELISA) for the detection of antibody to malignant catar...
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ژورنال
عنوان ژورنال: Journal of Veterinary Medical Science
سال: 1996
ISSN: 0916-7250,1347-7439
DOI: 10.1292/jvms.58.1